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So far you have tested the effect of substrate (H₂O₂) concentration on the catalase reaction. Temperature: To measure the impact of temperature on enzyme efficiency you have two options. If your results are different from your hypothesis, why might they differ? This option tends to yield the best results. IntroductionHave you ever wondered how all the food that you eat gets digested?

Catalase Enzyme Lab Answer Key Of Life

Plastic Cups, 16 oz (5). List the factors that can affect the rate of a chemical reaction and enzyme activity. The given chart and graph show the different concentration levels of the substrate that were used, along with the time it took for the reaction to occur. Collect the paper discs out of your hole puncher (or hit up the copy center at your school).

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The higher temperature decreased stem diameter, but room temperature had thicker stems. Repeat the catalase reaction but this time vary conditions such as the pH by adding vinegar or baking soda, or change the reaction temperature by heating the solution in the microwave. One important thing to know about enzymes is that they are proteins. The objective of this lab was to develop a protocol to investigate the effect of an environmental variable on the catalytic function of an enzyme. In this science activity you will investigate one of these enzymes, called catalase, to find out how it helps to protect your body from cell damage. When there were zero concentrates of hydrogen peroxide, meaning the solvent was only water, there was no reaction with the catalase. You will need to use your scientific method knowledge in today's lab in creating hypotheses for each experiment, devising a protocol to test your hypothesis, and analyzing the results. Wash the test-tubes with water and drain to remove the excess water. Recommended textbook solutions. Hydrogen peroxide is a toxic product of many chemical reactions that occur in living things. The experimental variable (independent variable) is the aspect that varies between the experimental groups. Catalase enzyme lab answer key of life. Looking at all your results, what do you think is the limiting factor for the catalase reaction in your cups? The catalase needs its specific substrate to bind with in order for a reaction to occur. Results: This section provides a written description of the data in paragraph form.

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Also at the absorbance of a slope of 0. List 2 controlled variables in the experiment. Are there any conditions that eliminate the catalase activity? The functionality of an enzyme is determined by the shape of the enzyme.

Enzyme Action Testing Catalase Activity

How does the blended liver look after heating? Other chemical reactions need energy, without which they would proceed very slowly or not at all. Oxidative damage is damage to cells or other molecules in the body caused by oxidative compounds. To show the similarity between enzymes and chemical catalysts, the teacher may wish to demonstrate (or ask the class to perform as part of the class experiment) the catalytic decomposition of hydrogen peroxide solution by manganese(IV) oxide (HARMFUL – see CLEAPSS Hazcard HC060). Prepare a line graph of catalase activity as influenced by temperature. Recent flashcard sets. Put one drop of the mixture on a clean part of the large plate and add one drop of hydrogen peroxide to it. The liver maintains a neutral pH (about pH 7), which is easiest for its enzymes, such as catalase, to work in. What might explain your results? Catalase enzyme lab answer key image. The term may have been met previously in biological topics, but the notion that they act as catalysts and increase the rate of reactions may be new. What is the color and consistency of this mixture? The effect of temperature on the activity of catalase.

These should be general, not specific, statements that are possible answers to your questions. The enzyme catalase quickly breaks down hydrogen peroxide into water and oxygen. Hydrogen peroxide solution, '5 volume'. Catalase Enzyme Lab Flashcards. If you have an excess of enzyme but not enough substrate, the reaction will be limited by the substrate availability. Make sure your instructor checks your procedures before you continue.

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